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In general, iron overload in cells, decreased GPX4 and xCT expression, activation of acylCoA synthetase long-chain family member-4 (ACSL4) and lysophosphatidylcholine acyltransferase-3, and an increase in lipid peroxidation are the four main mechanisms that induce ferroptosis [82,83,84]
Purified GST protein is also offered as a positive control for purification and Western blotting
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