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The β-arrestin1 siRNA (5′-CCAGCUCAACAUUCUGCAATT-3′) and β-arrestin2 siRNA (5′-GCUUGUGGAGUAGACUUUGTT-3′) purchased from GenePharma (Shanghai, China) were transfected into astrocytes using Lipofectamine 3000 reagent (Invitrogen, L3000015) according to the manufacturer’s protocol
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