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Growth hormone and somatostatin

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A 10 μl reaction system was assembled with 25 nM lysine methyltransferase enzyme (or enzyme complex), 0.5 µM SAM, 100 nM mononucleosome (EpiCypher), MTase-Glo reagent (1×) and a series of diluted NSD2i in reaction buffer (containing 50 mM Tris pH 8.0, 20 mM KCl, 5 mM MgCl 2 and 10% glycerol) in a white 384-well microplate (Corning)