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To remove 4HT, cells were washed five times with 4HT-free media with the last two washes 30 minutes each before re-seeding at 300,000 cells per mL

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GLUTAMATE–GLUTAMINE CYCLE Glutamate–glutamine cycle suggests that glutamatergic neurons release glutamate to synapses through vesicles, and most of the glutamate in synapses is transported by glutamate transporters on astrocytes and converted into glutamine in cells

While our body can produce L