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Readouts should triangulate source and compartment: quantify superoxide with DHE-HPLC (particularly informative for NOX2), track H 2 O 2 with compartment-targeted HyPer sensors and couple these to endothelial function tests (e.g., flow-mediated dilation or wire myography), while avoiding naïve reliance on raw probe fluorescence (see “Small-molecule fluorescent/chemiluminescent probes” section) that cannot disambiguate species or sources (Pak et al
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