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Bone degeneration and its recovery in SMP30/GNL-knockout mice

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Glutathione peroxidase (GPX) GPX was measured adding cell extract to 50 mmol/l potassium phosphate pH 7.0 containing 1 mM EDTA, 1.5 mM sodium azide, 0.15 IU of purified glutathione reductase from bakers' yeast, 0.45 mM GSH, 0.2 mM NADPH, 1.2 mM cumene hydroperoxide One unit of GPX was defined as the amount of enzyme which produces 1 µmol of GSSG (oxidized glutathione)/min at 25°C