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Immunoblotting To profile protein lipoylation and lysine glutarylation, 0.5 × 10 6 HeLa cells or 1 × 10 6 T cells were lysed in SDS loading buffer (2% (wt/vol) SDS, 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM dithiothreitol, 10% (vol/vol) glycerol and 0.2% (vol/vol) benzonase nuclease (Sigma-Aldrich, E1014)) and heated at 90 °C for 5 min

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Second, in the human-based research that was conducted, only male volunteers were used in the analysis, resulting in information about a subset of the population