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Since we found that fork reversal induced by HRASV12 or cyclin E1 overexpression is caused by R-loops, we sought to test whether PARP inhibition with olaparib or siRNA-mediated depletion of ZRANB3 induces unrestrained replication fork progression in U2OS cells transduced with HRASV12 or cyclin E1 retroviral vectors

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There is, therefore, interest in developing and leveraging “-omics” technologies (lipidomics, proteomics, transcriptomics) to facilitate identification of potentially beneficial drug synergies or of detrimental drug interaction based on cheaper short-term biomarker studies