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Additional investigations unveiled that this subset of Purkinje cells situated within the vermal PL region exhibited relatively heightened expression of genes associated with “glycolysis/gluconeogenesis,” “pentose phosphate pathway,” “fructose metabolism,” and “oxidative phosphorylation,” suggesting these cells by default have relatively greater activities and reliance in the central carbon metabolism network (Fig

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Subsequently, sterilized HAAT extract (prepared by dissolving 2 g of HAAT in 1 mL of culture medium) was added to the wells