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The following primers and probe were used for the quantification 75 : CS- gag forward primers: 5′- GACTAGCGGAGGCTAGAAGGAGAGA’-3′ CS- gag reverse primers: 5′- CTAATTTTCCSCCDCTTAATAYTGACG’-3′ CS- gag FAM/BHQ Probe: 5′-AT + G + GGT + GC + GAGA-3′ For some participants, primers and/or probes were customised based on the gag sequence determined in CA-RNA (Supplementary Table 3)

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This data indicates that the microstructures of the ECM could directly affect glutathione availability