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AOD9604 – Lumen Sciences (PC)
Generation of CRISPR KO strains To generate a knockout of glutathione reductase (GSR) in HEK293 cells, a guide RNA sequence targeting exon 1 was cloned into the pSpCas9(BB)-2A-GFP (PX458) vector, gifted from Feng Zhang (Addgene plasmid #48138, Watertown, MA, United States) [75]
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B12 and folate work together in several metabolic pathways
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