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meliloti , and Brucella melitensis , as well as cobyrinate a, c -diamide synthase CbiA from Salmonella typhimurium , Bacillus megaterium , and Methanococcus jannaschii were all reported could convert HBA into HBAD in natural metabolic pathway (but CbiA enzymes prefer to catalyze the derivant of HBA, cobyrinate, to form cobyrinate a, c -diamide in natural anaerobiotic metabolic pathway), by catalyzing the amidation at the a and c side chains of the corrin ring using l-glutamine as amide donor 31,32,33,34,35,36,37,38
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