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Glutathione reductase reaction | MedLink Neurology
After washing the membrane 3 × 5 min with TBST, Anti-biotin HRP-linked Antibody (Cell Signaling, 7075S, RRID:AB_10696897) was added to the membrane (1:1500 dilution) and incubated for 1 h with agitation
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A poorly spun preparation may barely exceed baseline — and will do correspondingly little
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