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conversely, if you have used the direct IP method, try the indirect IP method -Check the amount of beads and sample volume with respect to the capacity of the different beads provided in the package inserts, and increase the amount of beads or the concentration of your antibody during coupling -Increase the incubation time -If using a commercially sourced antibody, confirm it is validated for IP -Try another antibody What should I do when the antibodies coupled to Dynabeads Protein A or Protein G magnetic beads have lost function after crosslinking
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Stimulation of collagen formation by insulin and insulin-like growth factor I in cultures of human lung fibroblasts
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