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Michaelis–Menten kinetics were calculated by first obtaining the concentration of TNB formed under the reactions ( c = A / bE ), where c is the concentration, A is absorbance, b is the path length (1 cm) and E is the molar absorptivity (molar extinction coefficient of TNB is 14,150 M −1 cm −1 (ref
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New York, NY, USA: 2006
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