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aureus , fragments of 1 kb corresponding to the upstream and downstream flanking regions of murI and dat genes were amplified by PCR using primers UP_ murI (MluI)/UP_ murI (NotI), DOWN_ murI (NotI)/DOWN_ murI (BglII), UP_ dat (MluI)/UP_ dat (NotI) and DOWN_ dat (NotI)/DOWN_ dat (BglII), and next digested by the restriction enzymes indicated within brackets

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It is recommended to use it regularly, preferably morning and evening, for best results