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on glutathione sepharose 4b ge healthcare From Protein purification
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As expected, FSP1 was a top hit in the transcriptional screen (BFP), exhibiting strong enrichment in BFP low cells because of the disruption to FSP1 expression (Fig
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The MD simulations supported this as the disulphide bond was cleaved as a consequence of a subsequent OH addition to the resulting glutathionyl radical (GS•)
Purified GST protein is also offered as a positive control for purification and Western blotting
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