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The bile acids were detected using the following multiple reaction monitoring conditions: cholic acid (m/z 407→341, collision energy 36 V), chenodeoxycholic acid (m/z 391→391, CE 30 V)
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Preparation Condition 2 (EDTA → deproteinization → −80 o C): Anticoagulants used during collection impacts measures of GSH Suggesting the anticoagulant used during sample collection significantly alters plasma GSH, we found lower levels of free GSH, higher levels of GSSG, and a lower ratio of free GSH to GSSG in the heparinized plasma (Preparation Condition 1) compared to the EDTA-treated plasma (Preparation Condition 2)
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