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Each compound was evaluated for its growth inhibitory activity against HCT116 cells (ATCC) by the following method: the HCT116 cells were inoculated at 900 cells/40 µL/well to a 384-well white plate (Greiner Bio-one) and maintained in an assay medium (RPMI medium (Wako Pure Chemical Industries, Ltd.) containing 2% fetal bovine serum, 50 units/mL penicillin and 50 µg/mL streptomycin (Invitrogen Corp.))
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