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RNA Extraction and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted from cellular and cerebral samples using TRIzol reagent (Invitrogen, Carlsbad, CA, United States)
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Treating these islets with GSH led to an improvement in their functionality and also alleviated oxidative damage markers [36], suggesting that reducing OS in islets could be a potential target for treating diabetes
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