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Following the manufacturer's instructions, siRNAs were transiently transfected into the THP-1 cell line using Lipofectamine 3000 reagent (Invitrogen)
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Once activated, platelets secrete various immunomodulatory and microbicidal factors stored in their alpha-granules, including defensins, thrombocidins, kinocidins, and other antimicrobial peptides (85)
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