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Cells were then washed three times and covered with a coverslip using Prolong Gold antifade reagent (Thermo Fisher Scientific, P36930) and visualized using a Leica 8 STELLARIS Falcon at ×63 magnification

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ChIP was incubated with the appropriate antibodies against mono-methyl-histone H3 (Lys4) (H3K4me1, #5326), acetyl-histone H3 (Lys27) (H3K27ac, #8173), and FLAG (#14793) from Cell Signaling Technology (Shanghai, China), followed by a pull-down assay using protein A/G-conjugated beads