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Gene editing of k13 A212T by CRISPR/Cas9 CRISPR/Cas9 editing was performed to introduce or remove the A212T mutation from the k13 locus in Cam3.II clones, using the all-in-one plasmid pDC2-cam-coSpCas9-U6-gRNA-h dhfr as previously described ( 63 ), with the following modifications
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