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After 72 or 48 hours (experiments shown in Figures 1H-1J, S1J-S1L, and Figures 4B-4D, S4A, respectively) metabolism was quenched and samples prepared for LC-MS measurements as described for SAM, SAH, cystathionine, GSH and GSSG metabolites extraction and LC-MS/MS analysis, and using −80°C 80:20 methanol:water as extraction solvent
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