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Steps to start your routine: Step 1: Consult with a healthcare professional to determine if you have any specific mineral deficiencies
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To achieve the final volume of 650 µL for each sample, a phosphate buffer of 0.1 M and pH 7.5, containing 0.05 mM of 2-mercaptoethanol (Sigma-Aldrich, Darmstadt, Germany) was added in appropriate amounts
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