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The consistent decrease in increased MDA values in the blood, brain, heart, liver, kidney, and gastrointestinal tract is the next piece of evidence that BPC 157 therapy exerts its pleiotropic beneficial effects in all rats that have intra-abdominal hypertension (grade III and grade IV), decompression, and reperfusion in a particular way [133,134]

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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 µl of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 °C