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Cell lysates were transferred to Eppendorf tubes and debris was removed by centrifugation at 14000 rpm for 20 min at 4°C
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Analytical Purity Verification As with other synthetic research peptides, BPC-157 batches are commonly verified using a combination of high-performance liquid chromatography (HPLC) and mass spectrometry to confirm both sequence identity and the absence of synthesis byproducts such as truncated or incompletely deprotected fragments
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