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Cells were lysed with 1% Triton X-100 in PBS supplemented with protease inhibitors, biotinylated proteins captured with streptavidin-Sepharose for 1hour at 4°C and eluted using 100mM DTT after extensive washing
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Transcription factor nrf2 plays a pivotal role in protection against traumatic brain injury-induced acute intestinal mucosal injury in mice
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