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Previous work on H1.0b X.lae -bound hexanucleosomes showed that arrays imaged at low (5–10 mM) NaCl concentration display a zigzag configuration, whereas those in 0.35 or 0.6 mM MgCl 2 exhibit a two-start helical arrangement of stacked nucleosomes, adopting either a flat or twisted conformation, with the twisted form predominating at the higher Mg 2+ concentration 34 (Fig
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