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Animals began the study on a diet containing 2% EtOH (v/v) (10.8% EDC) and the amount of EtOH was increased 1% weekly until it reached 5% (v/v) (26.9% EDC) and the animals were maintained on 5% EtOH (v/v) for the remainder of the study

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These changes allow researchers to study amylin receptor signaling and related metabolic pathways over longer timeframes than would be possible with endogenous amylin alone [1]