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The tissues were rinsed with PBS and incubated with Alexa Fluor488 chicken anti-rabbit (1:1000, Life Technologies) for one hour at 37 °C and then rinsed with PBS and incubated with Alexa Fluor488 goat anti-chicken antibody (1:1000, Life Technologies) and Alexa Fluor568 goat anti-mouse antibody (1:1000, Life Technologies) for another hour at 37 °C

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The mitochondria-targeting iridium(III) complex IrL2 (Figure 29) showed promising cytotoxicity against A2780 cells, triggering heme oxygenase-1 (HMOX1)-dependent ferroptosis

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