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Ubiquitination is a highly prevalent posttranslational protein modification involving a three-step enzymatic cascade, which includes E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin ligase) enzymes transferring ubiquitin from its C-terminal glycine to the ε-amino group of a lysine residue on the substrate [155, 156]
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