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1.2) using 3 mW of 405 nm laser power, 0.77 mW of 488 nm laser power, 0.7 mW of 561 nm laser power and 125 mW of 647 nm laser power, a pinhole aperture of 12.77 mm and both PMT and GaAsP detectors, all of which were controlled using NIS Elements C software v4.50 (Nikon)