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Cells (1 × 10 6 ) were transduced with 0.5–1 ml of viral supernatant in 2 ml of growth medium with 10 mM HEPES and 8 µg ml −1 polybrene in six-well format and centrifuged at 2,600 rpm for 2 h at 35 °C
peptide signalling research
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Kontogiorgis C, Detsi A, Hadjipavlou-Litina D
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