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monocytogenes macrophage infection 0.5 × 10 6 primary murine macrophages from WT C57BL/6 mice were plated in BMM media (DMEM with 10% heat inactivated fetal bovine serum, 1mM L-glutamine, 2mM sodium pyruvate and 10% L929-conditioned medium) in a tissue culture treated 24-well dish (Greiner Bio, #662165) with the addition of 100ng recombinant murine IFN-γ (Peprotech, #315-05) for 18 hours