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HeLa Tet-Off and Hela Tet-On Advanced cell lines (Clontech), TIG-7, a normal human fibroblast cell line, HEK293T, a human embryonic kidney cell line expressing a mutant version of the SV40 large T antigen, were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Fujifilm Wako Pure Chemical) supplemented with 10% (v/v) FBS and 1% (v/v) penicillin-streptomycin under the same conditions

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The rax1-1 mutant is characterized by an enhanced APX2 gene expression due to a lesion in the gene encoding GSH1 concomitantly with a 50% reduced GSH content

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