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The next day, cells were treated with ferrostatin-1 or TEMPO (Sigma-Aldrich 214000-1 G) or mitoTEMPO for 24 h, followed by RSL3 or ML162 for 48 h in 100 µL cell culture medium

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Chemical binding of afatinib and ibrutinib to GSH, respectively b) Application of GSH chemical binding model Screening for active "warheads" of covalent drugs Covalent drugs are small molecular compounds with electrophilic groups that can bind to target proteins, thereby inhibiting their biological functions or targeting them for degradation [8]