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The protein bands were detected using ECL detection reagents (Thermo Scientific) and exposed on blue X-ray film (Phenix Research Products, Candler, NC)

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When blood flow to an organ does not limit elimination (see below), the relation between elimination rate and concentration (C) is expressed mathematically in equation (5): The maximum elimination capacity is V max , and K m is the drug concentration at which the rate of elimination is 50% of V max