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Briefly, the intensity, as determined by the area under the peak, of all peaks relating to SOD1 monomers ( I M ) and dimers ( I D ) were summed to give total signal intensity ( I T ): The proportion of monomer signal ( P M ) was determined by dividing the intensity of the monomeric species ( I M ) by the total signal intensity ( I T ): The concentration of monomer at equilibrium ([ M ] eq ) was determined by multiplying the proportion of monomer signal ( P M ) by the total protein concentration in micromolar ([ P 0 ]): The concentration of dimer at equilibrium ([ D ] eq ) is defined as K d values were derived from the slope of a linear regression fit of the plot of [ D ] eq against [ M ] 2 eq (fitting performed with Prism 5.0, GraphPad Software, Inc.) (Supplementary Fig
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4.2.2 Tolerance to Heat, Oxygen, and Light SAG exhibits enhanced thermal stability compared to GSH, tolerating standard manufacturing temperatures with minimal degradation
